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MB Sample ID: SA189635
| Local Sample ID: | 20190313-QC3 |
| Subject ID: | SU002106 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | NF54 attB |
| Gender: | Not applicable |
| Cell Counts: | 1x10^8 |
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Collection:
| Collection ID: | CO002099 |
| Collection Summary: | Plasmodium falciparum NF54 attB parasites containing different genetic backgrounds were cultured in RPMI 1640 medium and magnetically enriched to increase the infected to uninfected RBC ratio. Following hemocytometer counts, 1x10^8 parasites were measured per condition into 5 mL of total media for 2.5 hours in the presence of the universally labelled carbon-13 metabolite (glucose, glutamine, or acetate). Medium was aspirated until 1 mL remained on each sample, the sample was transferred to a micro-centrifuge tube, spun, and the medium was aspirated. |
| Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Sample Type: | Cultured cells |
| Collection Location: | Millenium Science Complex, University Park, Pennsylvania |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 mL eppendorf |
| Storage Vials: | 1.5 mL eppendorf |
| Collection Tube Temp: | On ice |
| Tissue Cell Quantity Taken: | 1x10^8 cells per sample in 1 mL total volume |
