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MB Sample ID: SA193777
Local Sample ID: | 2 - CA1 2 |
Subject ID: | SU002137 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Collection:
Collection ID: | CO002130 |
Collection Summary: | Samples were analyzed for 180 metabolites using the AbsoluteIDQ® p180 kit from Biocrates Life Sciences AG at the Analytical Facility for Bioactive Molecules (The Hospital for Sick Children, Toronto, Canada). Collected cell pellets were resuspended in 15 % ice-cold 10 mM Phosphate Buffer (PB) + 85 % EtOH as per AbsoluteIDQ® p180 kit protocol. Resuspended cells were subjected to three rounds of sonication and rapid freeze thawing. Final suspension was then centrifuged at 20,000 x g for 10 minutes at 4°C. A small aliquot was taken for protein assay, and the remainder of the supernatant was used for metabolic analysis. Samples, standards, and controls (10 mL each) were added to the Biocrates 96-well filter plate with internal standard, dried under nitrogen, derivatized with phenyl isothiocyanate, and then extracted with methanol, as per kit instructions. |
Sample Type: | human cells |