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MB Sample ID: SA193916
| Local Sample ID: | CTL-9_R1- |
| Subject ID: | SU002140 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002133 |
| Collection Summary: | Lung and skeletal muscle (TA and bulk muscles from the upper arm) were collected upon necropsy and imaged for mCherry+ 4T1-SkM lesions (Zeiss AxioZoom). Once a mCherry+ lesion was identified, the region was excised, leaving as little tissue margins as possible. The sample was weighed and then flash frozen using LN2. Healthy lung and skeletal muscle samples were collected through the same process, without the mCherry imaging step. 4T1-parental and 4T1-SkM cells were plated in 6-well plates at an initial density of 50,000 cells/well and collected 48h later (final density ~275,000 cells/well). Wells were washed twice with 1x PBS and cells were scraped into 1.5 mL microcentrifuge tubes and spun at 16,000 x g for 3 minutes. Samples were then flash frozen and stored at -80°C until ready to process. |
| Sample Type: | Lung |
