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MB Sample ID: SA196505
| Local Sample ID: | Fer1-1 |
| Subject ID: | SU002164 |
| Subject Type: | Cultured cells |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Cell Biosource Or Supplier: | American Type Culture Collection (ATCC) |
| Cell Strain Details: | H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. |
| Cell Passage Number: | 3-10 |
| Cell Counts: | 80-90% confluency |
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Collection:
| Collection ID: | CO002157 |
| Collection Summary: | H9c2 cardioblasts were cultured according to the manufacturer’s recommendations (ATCC, Manassas, VA, USA) with minor modifications. Briefly, the cells were incubated in DMEM based modified media containing 4 mM L-glutamine, 4.5 g/L glucose, 1 mM sodium pyruvate, and 1.5 g/L sodium bicarbonate, pH 7.4, and supplemented with 10% fetal bovine serum and 1% antibiotic solution (HyClone, Logan, UT, USA) in a CO2 incubator containing 95% air and 5% CO2 at 37 ◦C. Cells with 80–90% confluence from passages 3–10 were used for experiments. All chemicals were purchased from Sigma-Aldrich (St. Louis, MO). H9c2 cardioblasts demonstrate similar to the primary cardiomyocytes hypertrophic response as well as mitochondrial metabolism and morphology. |
| Sample Type: | Cultured cells |
