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MB Sample ID: SA202333
| Local Sample ID: | P_2E7_1 |
| Subject ID: | SU002191 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
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Collection:
| Collection ID: | CO002184 |
| Collection Summary: | Heparin synchronized Pf3D7 and PfM17-HAglmS parasites were allowed to invade RBC for 4 h and any remaining schizonts were lysed by sorbitol synchronization. Parasite cultures were then treated for ~ 36 h with 2.5 mM GlcN or for 1 h with 3 at 10x the EC50 (Pf3D7 only) or left untreated. Parasites were harvested at developmentally similar timepoints by centrifugation at 900 g for 5 min and then resuspended in 10 mL of chilled PBS. Parasite metabolism was quenched by cooling samples to between 3-5°C in an ethanol-dry ice bath. The rest of the preparation was performed at 4°C. Parasites were magnet purified on a VarioMACS column and 3x107 parasites were used for downstream analysis. All samples were centrifuged at 650 g for 3 min, the supernatant was removed, and the pellet washed in 500 µL of ice-cold PBS. Samples were again centrifuged at 650 g for 3 min and pellets were resuspended in 150 µL of ice-cold extraction buffer (100% methanol) and quickly resuspended. The samples were then incubated on a vortex mixer for 1 h at 4°C before being centrifuged at 17,000 g for 10 min; from this 100 µL of supernatant was collected and stored at -80°C until analysis. For each sample, another 10 µL was collected and pooled, to serve as a quality control (QC) sample. |
| Sample Type: | Blood (whole) |
