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MB Sample ID: SA202460
| Local Sample ID: | ChR_2WPC_PS_POS_(2) |
| Subject ID: | SU002196 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002189 |
| Collection Summary: | Three-month-old Thy1-Chr2-eYFP and C57BL6/J mice were anesthetized by intraperitoneal injection of Ketamine/Xylazine cocktail. Before crush, eyes received topical anesthesia using 0.5/% proparacaine. A 1 mm hole in the superior-temporal conjunctiva was made, the muscle tissue was separated, and the optic nerve was exposed. The optic nerve was then crushed using Dumont #5 forceps (Fine Science Tools, Foster City, CA, USA) at approximately 0.5-1 mm behind the globe without damaging retinal vessels or affecting the blood supply. Mice of either sex were randomly assigned into either the optogenetic stimulation group or the control group. The mice in the optogenetic stimulation group received blue light stimulation (~470 nm) at 1 HZ frequency while the mice in the control group were kept in a normal 12h light/dark environment. The mice were kept in the stimulated environment one or two weeks after crush according to the experimental design. |
| Sample Type: | Eye tissue |
