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MB Sample ID: SA204321
| Local Sample ID: | BM_supernatant_5FU_2 |
| Subject ID: | SU002210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002203 |
| Collection Summary: | 5×10^5 indicated cells were incubated with 100 µM stable [13C,15N] amino acids (MSK-A2-US-1.2, Cambridge Isotope Laboratories) for indicated time and centrifuged at 500 g for 5 min at 4 °C. The pelleted cells were extracted in 500 µl ice-cold Acetonitrile: Isopropyl Alcohol: water (3:3:2 v/v/v) and aliquoted as three technical replicates. The extracts were vortexed for 5 min at 4 °C and centrifuged at 14,000 g for 2 min at 4 °C. The supernatants were dried by vacuum spin for subsequent derivatization and stored at -20 ℃. |
| Sample Type: | Bone marrow |
