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MB Sample ID: SA211371
| Local Sample ID: | LV18_01 |
| Subject ID: | SU002291 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002284 |
| Collection Summary: | Cells were seeded in 6-well plates and collected the following day at 70% confluency. Cells were washed at room temperature with PBS twice and then kept on cold bath with dry ice and methanol. Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% ultrapure water, 5 µM final concentration valine-d8) was added to each well following the proportion of 0.5 ml of extraction solution per million cells. The extracts were scraped and mixed at 4°C for 15 min. After final centrifugation at max speed for 15 min at 4°C, the supernatants were transferred into LC-MS vials. |
| Sample Type: | kidney epithelial cells |
