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MB Sample ID: SA212025
| Local Sample ID: | MS57_060 |
| Subject ID: | SU002308 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002301 |
| Collection Summary: | 2x105 cells were plated onto 6-well plates (5 replicates for each cell type). The day after, the medium was replaced with fresh one containing 13C5 glutamine in the presence of vehicle (DMSO) or GLS inhibitor CB-839 (100nM, 500nM or 1μM) and further incubated for 23h. Before extraction, cells were counted using CASY cell counter (Omni Life Sciences) using a separate counting plate. After that, cells were washed at room temperature with PBS twice and then kept in a cold bath with dry ice and methanol before adding the metabolite extraction solution. |
| Sample Type: | Cultured cells |
