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MB Sample ID: SA212235
| Local Sample ID: | MS52-04 |
| Subject ID: | SU002311 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002304 |
| Collection Summary: | 2x105 cells were plated onto 6-well plates (5 replicates for each cell type). The day after, the medium was replaced with fresh one containing 13C6-Leucine and 13C6-Isoleucine (obtained from Cambridge Isotopes Laboratories) for 10 mins, 1 hour, 3 hours in Plasmax media. Before extraction, cells were counted using CASY cell counter (Omni Life Sciences) using a separate counting plate. After that, cells were washed at room temperature with PBS twice and then kept in a cold bath with dry ice and methanol before adding the metabolite extraction solution. |
| Sample Type: | Cultured cells |
