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MB Sample ID: SA217761
| Local Sample ID: | 0hpa_M |
| Subject ID: | SU002357 |
| Subject Type: | Other organism |
| Subject Species: | Xenopus tropicalis |
| Age Or Age Range: | Stage 41-43 |
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Collection:
| Collection ID: | CO002350 |
| Collection Summary: | Tadpoles were anesthetized with 0.05% MS-222 in 1/9x MR and tested for response to touch prior to tissue collection. For uninjured tips (Tips), a sterilized scalpel was used to amputate 500µm of the posterior tip. For 0 hours post amputation (hpa) samples, the posterior third of the tail was amputated followed by collection of tail tissue 250µm anterior to the initial wound. For 3 and 24hpa, 250µm of tissue, including the regenerating tissue, was collected. 10 replicates of 25 tails (8 replicates for the 24hpa timepoint) were collected. For each sample, media was removed, and samples were frozen on dry ice within 5-8 minutes from the first amputation. |
| Sample Type: | Tails |
