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MB Sample ID: SA246076
| Local Sample ID: | 07_KO_Rapha1 |
| Subject ID: | SU002548 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
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Collection:
| Collection ID: | CO002541 |
| Collection Summary: | PTCs were isolated as previously described(PMID: 32484794). Briefly, kidneys were excised in ice-cold HBSS buffer. The kidneys were sliced and chopped into pieces (~0.5–1 mm) on ice using a surgical scalpel. The chopped kidneys were transferred into an HBSS solution containing 1 μg/μL collagenase/dispase (Sigma Aldrich, Cat. # 10269638001) and incubated for 25 minutes at 370 C. The cells were filtered through a 40-μm nylon cell strainer (Corning, Cat. # 431750) and washed twice with cold HBSS. For PTC isolation, the cells were stained with PE-conjugated antiCD133/prominin-1 antibody (Invitrogen, Cat. # 12-1331-82) according to the manufacturer's instructions. PE+ cells were isolated by Aria III flow cytometry-based cell sorting. |
| Sample Type: | Proximal tubular cells |
