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MB Sample ID: SA248782
| Local Sample ID: | MSL-7 |
| Subject ID: | SU002577 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
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Collection:
| Collection ID: | CO002570 |
| Collection Summary: | Spleens of C57BL/6 mice were homogenized to a single cell suspension by mashing the spleen through a 70 uM cell strainer, and the erythrocytes were lysed with ACK (ammonium-chloride-potassium) lysis buffer for 3 min at RT. CD8 T cells were sorted with a CD8-negative selection Stemcell isolation kit following manufacturer instructions. Cells were washed with PBS at 1200RPM, 4degC for 10 minutes and pellets were flash frozen. |
| Sample Type: | Spleen; T-cells |
