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MB Sample ID: SA257047
| Local Sample ID: | 10165_Prepartum_Con |
| Subject ID: | SU002657 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Weight Or Weight Range: | 700 kg |
| Gender: | Female |
| Animal Housing: | Free-stalls |
| Animal Feed: | TMR |
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Collection:
| Collection ID: | CO002650 |
| Collection Summary: | All cows had blood collected in the prepartum period (-14 DRP), calving (first 24h after calving), and at diagnosis (day of metritis diagnosis). Blood was sampled from the jugular vein using a 20-gauge x 2.54-cm needle and 10-mL evacuated tube containing lithium heparin (Vacutainer, Becton, Dickinson and Company, Franklin Lakes, NJ, USA). After collection, the blood tubes were placed on ice and transported to the laboratory within 2 hours. Once in the laboratory, the blood tubes were centrifuged at 4000 g, 4 °C, for 10 min, and the plasma was stored at -80 oC for further characterization of the plasma metabolome. The frozen plasma was submitted to the University of California’s West Coast Metabolomics Center in Davis, CA for metabolome analysis. Samples were analyzed by blinded technicians using untargeted gas chromatography with time-of-flight mass spectrometry (GC-TOF-MS) in a single batch. |
| Sample Type: | Blood (plasma) |
