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MB Sample ID: SA001764
| Local Sample ID: | Control_4S |
| Subject ID: | SU000057 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Genotype Strain: | N2 |
| Age Or Age Range: | Young Adult |
| Gender: | Hermaphrodite |
| Species Group: | Invertebrate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000060 | AN000061 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 1250 | Thermo Accela 1250 |
| Column | Thermo Scientific Gold aQ | Thermo Scientific Gold aQ |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
MS:
| MS ID: | MS000041 |
| Analysis ID: | AN000060 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were analyzed using a mass range of m/z 70-800 in positive and negative ionization mode, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with an Open Accela autosampler and an Accela 1250 pump (San Jose, CA). The Q-Exactive was equipped with a heated electrospray ionization (HESI) source, which operated at a spray temperature of 500 °C, a spray voltage of 3 kV, and sheath and auxiliary gas flow rates of 60 and 10 arbitrary units, respectively. Features were extracted from the ms data using proprietary MATLAB IROA scripts. Ratios for each feature (heatshock/control) were calculated for each replicate. Ratios were normalized by setting the median log-fold change for each sample to zero |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 500 °C |
| Ion Spray Voltage: | 3 kV |
| Mass Accuracy: | 1.5 ppm |
| Scan Range Moverz: | 70-1000 |
| MS ID: | MS000042 |
| Analysis ID: | AN000061 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were analyzed using a mass range of m/z 70-800 in positive and negative ionization mode, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with an Open Accela autosampler and an Accela 1250 pump (San Jose, CA). The Q-Exactive was equipped with a heated electrospray ionization (HESI) source, which operated at a spray temperature of 500 °C, a spray voltage of 3 kV, and sheath and auxiliary gas flow rates of 60 and 10 arbitrary units, respectively. Features were extracted from the ms data using proprietary MATLAB IROA scripts. Ratios for each feature (heatshock/control) were calculated for each replicate. Ratios were normalized by setting the median log-fold change for each sample to zero |
| Ion Mode: | NEGATIVE |
| Ion Source Temperature: | 500 °C |
| Ion Spray Voltage: | 3 kV |
| Mass Accuracy: | 1.5 ppm |
| Scan Range Moverz: | 70-1000 |
