Return to study ST000115 main page
MB Sample ID: SA006130
| Local Sample ID: | D-25 |
| Subject ID: | SU000134 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 13-wk-old |
| Gender: | Male |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000195 | AN000196 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | ||
| Chromatography system | ||
| Column | ||
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex API 5000 QQQ | Thermo TSQ Quantum Ultra |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | uM | uM |
MS:
| MS ID: | MS000158 |
| Analysis ID: | AN000195 |
| Instrument Name: | ABI Sciex API 5000 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Briefly, 50 ul of plasma was spiked with heptadecanoate internal standard (ISTD) and analyzed with Applied Biosystems (Foster City, CA) API5000 mass spectrometer coupled with a Cohesive (Franklin, MA) TX2 liquid chromatography system. Concentration of individual FFA was measured against a six-point standard curve prepared for each analyte. Both the ISTD and individual FFA standard curves were prepared in 2% fatty acid-free human albumin solution. All analytes were monitored as their [M - H]- ions. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000159 |
| Analysis ID: | AN000196 |
| Instrument Name: | Thermo TSQ Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | LCFa-CoA esters were estimated using the LC-MS/MS method (9). After extraction in the presence of internal standard (20 ng of heptadecanoyl-CoA), samples were analyzed by UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters Acquity UHPLC, C8 UPLC BEH column 2.1 × 150 mm, 1.7 um (Waters, Milford, MA) and TSQ Quantum Ultra triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]. All standard curves were prepared using chemicals from Avanti Polar Lipids. |
| Ion Mode: | POSITIVE |
