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MB Sample ID: SA086293
| Local Sample ID: | selenium-5 |
| Subject ID: | SU001289 |
| Subject Type: | Invertebrate |
| Subject Species: | Apis mellifera |
| Taxonomy ID: | 7460 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002035 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity I-Class |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE |
| Units | peak area |
MS:
| MS ID: | MS001887 |
| Analysis ID: | AN002035 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | We operated the MS in selected reaction monitoring mode with source and desolvation temperatures at 150° C and 500° C, respectively, with the desolvation gas set to 1000 L/hr, cone gas set to 150 L/hr and collision gas set to 0.15 mL/min. We used nitrogen gas for each step, except for the collision gas, which was argon, and we set capillary voltage to 1 kV in positive ion mode and 2 kV in negative ion mode. Similar to the untargeted methods, we generated a quality control sample by pooling equal aliquots of each sample and analyzed the QC sample every 3-4 injections to monitor system stability and performance. We processed the metabolite data (peak picking, alignment, deconvolution, integration, normalization, and spectral matching) with Progenesis Qi software (Nonlinear Dynamics, Durham, NC). We used Skyline software (MacLean et al. 2010) for data processing our targeted metabolites. |
| Ion Mode: | POSITIVE |
