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MB Sample ID: SA091061
| Local Sample ID: | 17_03_t_120 |
| Subject ID: | SU001319 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-27 |
| Weight Or Weight Range: | 58-92 |
| Height Or Height Range: | 172-190 |
| Gender: | Male |
| Human Race: | Caucasian |
| Human Trial Type: | Prospective randomized open label controlled intervention study |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | Non smoker |
| Human Alcohol Drug Use: | Not in |
| Human Nutrition: | Fasted for 12 hours before the first sample was obtained. |
| Human Inclusion Criteria: | Healthy (normal physical examination, electrocardiography, and routine laboratory values). |
| Human Exclusion Criteria: | Febrile illness during the 2 weeks before the endotoxemia experiment, taking any prescription medication, history of spontaneous vagal collapse, practicing or experience with any kind of meditation, or participation in a previous trial where LPS was administered. |
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Combined analysis:
| Analysis ID | AN002077 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Discovery HS F5-3 (15cm x 2.1 mm,3um) Supelco,Sigma Aldrich) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6540 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | relative abundance |
MS:
| MS ID: | MS001928 |
| Analysis ID: | AN002077 |
| Instrument Name: | Agilent 6540 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The 6540 QTOF/MS Detector (Agilent) operated in both positive and negative ESI mode in a detection range of 50 to 1700 m/z at 2 GHz in extended dynamic range. The DualAJS ESI source was set to the following parameters: Gas temperature 200 °C, drying gas 8 L/min, nebulizer 35 psig, sheath gas temp: 350 °C, sheath gas flow 11 L/min, VCAp 3500 V and nozzle voltage of 0 V. Online calibration of the instrument was performed throughout the data acquisition using Agilent ES-TOF Reference Mass Solution Kit. Chromatograms were generated by the LC-MS instrument in .d format. Raw data were converted into mzXML and chromatogram peaks were extracted using XCMS v1.42.0 (https://pubs.acs.org/doi/10.1021/ac051437y), which was optimized using the IPO R package (https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-015-0562-8) with the following settings: peakwidth=c(10, 70), ppm= 20, snthresh=10, mzdiff=0.0034, prefilter=c(3, 100), noise=100, gapInit=0.8448, gapExtend=2.0544, bw=5, mzwid=0.015, minfrac=0.5, max=50. All further analyses were performed in R programming language, Metaboanalyst 4.0 (https://www.nature.com/articles/nprot.2011.319), and GraphPad Prism version 5.0 (GraphPad Software). IDEOM software (http://mzmatch.sourceforge.net/ideom.php; https://academic.oup.com/bioinformatics/article/28/7/1048/210126) was used to eliminate noise and for putative peak annotation by exact mass within ±10 ppm against the Metabolomic Discoveries in house metabolite library (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0125044) in negative and positive ESI mode, respectively. Retention time prediction was applied to aid metabolite annotation. |
| Ion Mode: | UNSPECIFIED |
