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MB Sample ID: SA092069
| Local Sample ID: | Healthy2 |
| Subject ID: | SU001335 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 24-78 |
| Gender: | Male and female |
| Human Ethnicity: | caucasian |
| Human Inclusion Criteria: | tumour has not yet been treated with surgery, radiotherapy or chemotherapy; primary tumour |
| Human Exclusion Criteria: | concurrent other tumour |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002104 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | pmol |
MS:
| MS ID: | MS001955 |
| Analysis ID: | AN002104 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | POSITIVE AND NEGATIVE MODES IN ESI. MS AND MS2 MODES WERE USED. Different sample amounts were injected into the MS (described in the metadata), so before data analysis, the raw abundances in pmol need to be normalised to the amount injected. The raw data provided is in pmol per amount injected into the MS. As different amounts were injected you need to divide by the amount injected and the original concentration of the sample (which was 5mg/ml) to get pmol/mg of tissue weight. The detection limit given by Lipotype Ltd for the machine is 1pmol, and values less than that should also be removed from the raw data before analysis. When we normalised the data (normalised data not provided here), we required the presence of 80% or more 'non-zero' values in either group. Otherwise the feature was excluded. |
| Ion Mode: | UNSPECIFIED |
