Return to study ST001304 main page
MB Sample ID: SA094351
| Local Sample ID: | Ku80_3_shield |
| Subject ID: | SU001378 |
| Subject Type: | Cultured cells |
| Subject Species: | Toxoplasma gondii |
| Taxonomy ID: | 5811 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002172 | AN002173 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 RSLC | Thermo Dionex Ultimate 3000 RSLC |
| Column | ZIC-pHILIC,Merck | ZIC-pHILIC,Merck |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Signal Intensity | Signal Intensity |
MS:
| MS ID: | MS002021 |
| Analysis ID: | AN002172 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | DPSOHV ȝ/ ZHUH injected onto a Dionex RSLC U3000 LC system (Thermo) fitted with a ZIC-pHILIC column ȝP SDUWLFOH VL]H E\ PP 0HUFN DQG P0 DPPRQLXP FDUERQDWH $ DQG acetonitrile (B) were used as the mobile phases. A 30 min gradient starting from 80% B to 40% B over 20 min, followed by washing at 5% B for 3 min and re-equilibration at 80% B, was used. MS utilised a Q-Exactive Orbitrap MS (Thermo) with a heated electrospray source operating in positive and negative modes (rapid switching) and a mass resolution of 35,000 from m/z 85 to 1,050. Sample injections within the experiment were randomized to avoid any impact of systematic instrument drift on metabolite signals. Retention times for ~350 authentic standards were checked manually to aid metabolite identification. Metabolomics data sets were analysed using IDEOM. Raw files were converted to mzXML with msconvert, extraction of LC-MS peak signals was conducted with the Centwave algorithm in XCMS, alignment of samples and filtering of artefacts with mzMatch, and additional data filtering and metabolite identification in IDEOM. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002022 |
| Analysis ID: | AN002173 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | DPSOHV ȝ/ ZHUH injected onto a Dionex RSLC U3000 LC system (Thermo) fitted with a ZIC-pHILIC column ȝP SDUWLFOH VL]H E\ PP 0HUFN DQG P0 DPPRQLXP FDUERQDWH $ DQG acetonitrile (B) were used as the mobile phases. A 30 min gradient starting from 80% B to 40% B over 20 min, followed by washing at 5% B for 3 min and re-equilibration at 80% B, was used. MS utilised a Q-Exactive Orbitrap MS (Thermo) with a heated electrospray source operating in positive and negative modes (rapid switching) and a mass resolution of 35,000 from m/z 85 to 1,050. Sample injections within the experiment were randomized to avoid any impact of systematic instrument drift on metabolite signals. Retention times for ~350 authentic standards were checked manually to aid metabolite identification. Metabolomics data sets were analysed using IDEOM. Raw files were converted to mzXML with msconvert, extraction of LC-MS peak signals was conducted with the Centwave algorithm in XCMS, alignment of samples and filtering of artefacts with mzMatch, and additional data filtering and metabolite identification in IDEOM. |
| Ion Mode: | NEGATIVE |
