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MB Sample ID: SA095968
| Local Sample ID: | S5D |
| Subject ID: | SU001397 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002199 | AN002200 | AN002201 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | UNSPECIFIED | POSITIVE | UNSPECIFIED |
| Units | intensity | intensity | intensity |
MS:
| MS ID: | MS002045 |
| Analysis ID: | AN002199 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS002046 |
| Analysis ID: | AN002200 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002047 |
| Analysis ID: | AN002201 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column, coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source, operated in dynamic MRM mode. The source nitrogen gas temperature was set to 250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of 12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low pressure in positive and negative mode were 150 and 120. Check tunes were performed in wide, unit and enhanced modes prior to each experiment to confirm the performance of the mass spectrometer. The quadrupole was tuned to reference masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode; 112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode. |
| Ion Mode: | UNSPECIFIED |
