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MB Sample ID: SA096514
Local Sample ID: | OXGF14b-2-0h-2 |
Subject ID: | SU001405 |
Subject Type: | Plant |
Subject Species: | Oryza sativa Japonica Group |
Taxonomy ID: | 39947 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002219 | AN002220 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Waters CORTECS C18 (150 x 2.1mm,2.7um) | Waters CORTECS C18 (150 x 2.1mm,2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative content | Relative content |
MS:
MS ID: | MS002065 |
Analysis ID: | AN002219 |
Instrument Name: | Thermo Fusion Tribrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The spray voltage was set to 3500 V in the positive-ion modes, with the following ion-source properties: sheath gas, 45 Arbs; auxiliary gas, 10 Arbs; sweep gas, 0 Arbs; ion-transfer tube temperature, 320 °C; vaporizer temp, 350 °C. All FTMS data were acquired using the following conditions: detector type, orbitrap; orbitrap resolution, 120000; scan range was set to m/z 200-1200. The AGC was set at 5.0 e5 and the maximum injection time was set to 100 ms. RF lens was set to 60%, and the microscans was 1. data type, profile. All FTMS2 data were acquired using the following conditions: isolation mode, quadrupole; isolation window, 1 m/z; detector type, orbitrap; scan range, auto; AGC target, 5.0 e4; maximum injection time, 100 ms; microscans, 1; orbitrap resolution, 30000; first mass, 50 m/z; data type, profile. The HCD collision energy was set to 25% with ± HCD collision energy was set 5% in positive mode, and the HCD collision energy was set to 30% with ± HCD collision energy was set 5% in negative mode. All data was acquired by the Xcalibur 4.1 software (Thermo Fisher Scientific, USA). Thermo Scientific™ LipidSearch™ 5.0 software was used for lipidome data processing. |
Ion Mode: | POSITIVE |
MS ID: | MS002066 |
Analysis ID: | AN002220 |
Instrument Name: | Thermo Fusion Tribrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The spray voltage was set to -3000 V in the negative-ion modes, with the following ion-source properties: sheath gas, 45 Arbs; auxiliary gas, 10 Arbs; sweep gas, 0 Arbs; ion-transfer tube temperature, 320 °C; vaporizer temp, 350 °C. All FTMS data were acquired using the following conditions: detector type, orbitrap; orbitrap resolution, 120000; scan range was set to m/z 200-1200. The AGC was set at 5.0 e5 and the maximum injection time was set to 100 ms. RF lens was set to 60%, and the microscans was 1. data type, profile. All FTMS2 data were acquired using the following conditions: isolation mode, quadrupole; isolation window, 1 m/z; detector type, orbitrap; scan range, auto; AGC target, 5.0 e4; maximum injection time, 100 ms; microscans, 1; orbitrap resolution, 30000; first mass, 50 m/z; data type, profile. The HCD collision energy was set to 25% with ± HCD collision energy was set 5% in positive mode, and the HCD collision energy was set to 30% with ± HCD collision energy was set 5% in negative mode. All data was acquired by the Xcalibur 4.1 software (Thermo Fisher Scientific, USA). Thermo Scientific™ LipidSearch™ 5.0 software was used for lipidome data processing. |
Ion Mode: | NEGATIVE |