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MB Sample ID: SA113608
| Local Sample ID: | WT_DW_7 |
| Subject ID: | SU001473 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Samtako (Gyeonggi-do, Korea) |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002339 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Arbtrary unit |
MS:
| MS ID: | MS002181 |
| Analysis ID: | AN002339 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Mass spectra were recorded over a mass range of 85–500 m/z at an acquisition rate of 16 spectra/s. The temperatures of the ion source and transfer line of the TOF–MS were 250°C and 280°C, respectively. The electron ionisation energy was 70 eV.Chroma TOF Software C version (LECO) was used for the detection and deconvolution of peaks and mass spectra. BinBase was used for the identification and relative quantification of metabolites from preprocessed data by matching mass spectra and retention indices of peaks with the customised reference mass spectral and retention index libraries, acquired using authentic standards with identical data acquisition parameters for the Fiehn and NIST libraries. Peaks with mass spectral similarities above 700, in comparison to their authentic standards in the libraries, were regarded as authentic metabolites. Intensities of metabolites were reported as peak heights of their unique ion intensities. For management of missing values, the lowest background intensity was subtracted from the intensity of the quantified ion in its retention time region ± 5s, using MZmine. |
| Ion Mode: | POSITIVE |
