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MB Sample ID: SA124010

Local Sample ID:Jun17_2020_061
Subject ID:SU001525
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002425 AN002426
Analysis type MS MS
Chromatography type Normal phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Phenomenex Luna Silica (150 x 2.0mm,3um) Phenomenex Luna Silica (150 x 1.0mm,3um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Fusion Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE POSITIVE
Units pmol/mg pmol/umol

MS:

MS ID:MS002262
Analysis ID:AN002425
Instrument Name:Thermo Fusion Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Analysis of LC/MS data was performed using software package Compound Discoverer (ThermoFisher Scientific) with an in-house generated analysis workflow and oxidized phospholipid database.
Ion Mode:NEGATIVE
  
MS ID:MS002263
Analysis ID:AN002426
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:TAG cations were formed through molecular ammonium adduction (+NH4). Analysis was performed at a resolution of 140,000 for the full MS scan and 17,500 for the MS2 scan in a data-dependent mode. The scan range for MS analysis was 300–1200 m/z with a maximum injection time of 128 ms using one microscan. A maximum injection time of 500 ms was used for MS2 (high energy collisional dissociation (HCD)) analysis with collision energy set to 24. An isolation window of 1.0 Da was set for the MS and MS2 scans. Capillary spray voltage was set at 4.5 kV, and capillary temperature was 320 °C. Sheath gas was set to eight arbitrary units and the S-lens Rf level was set to 60. Standards for TAGs and their oxygenated metabolites were from Avanti Polar Lipids and Cayman Chemicals.
Ion Mode:POSITIVE
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