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MB Sample ID: SA163751
| Local Sample ID: | bileacid_Exp1_24_Prevention |
| Subject ID: | SU001825 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | GF 129SvEv background IL-10-deficient mice (Il10-/-); Il10-eGFP-reporter (Il10+/eGFP) mice (on a C57BL/6J background) |
| Age Or Age Range: | 8-12 week old |
| Animal Animal Supplier: | 8-12 week-age GF 129SvEv background IL-10-deficient mice (Il10-/-) were obtained from University of North Carolina National Gnotobiotic Rodent Resource Center; Il10-eGFP-reporter (Il10+/eGFP) mice on a C57BL/6J background mice were originally provided by Dr. C. L. Karp (Global Health, Bill & Melinda Gates Foundation, USA) |
| Animal Housing: | Germ-free and gnotobiotic conditions at National Gnotobiotic Rodent Resource Center, University of North Carolina |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002845 | AN002846 | AN002847 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7820A | Thermo Vanquish | Thermo Vanquish |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Orbitrap | Triple quadrupole |
| MS instrument name | Agilent 5977B | Thermo Q Exactive Orbitrap | Thermo Quantis QQQ |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | μmol/kg | ng/g | mg/g |
MS:
| MS ID: | MS002638 |
| Analysis ID: | AN002845 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | fullscan data with scan range of m/z 30-600; Peak integration was performed in MassHunter Qual. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002639 |
| Analysis ID: | AN002846 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Optimized PRM method scanning characteristic MS2 fragment ion for absolute quantitation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002640 |
| Analysis ID: | AN002847 |
| Instrument Name: | Thermo Quantis QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Optimized SRM method scanning characteristic MS2 fragment ion for absolute quantitation. |
| Ion Mode: | NEGATIVE |
