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MB Sample ID: SA163992

Local Sample ID:LN1_3-EV intensity
Subject ID:SU001832
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:SCC-9 and LN1
Cell Strain Details:Primary tumor (SCC-9) and metastatic (LN1) oral cancer cell lines

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Combined analysis:

Analysis ID AN002859
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent DB-35MS (30m x 0,32mm x 0,25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus HT TOF
Ion Mode POSITIVE
Units intensity log2

MS:

MS ID:MS002652
Analysis ID:AN002859
Instrument Name:Leco Pegasus HT TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:GC-TOF-MS data were obtained using a PAL-Combi XT autosampler (PAL System, Switzerland, http://www.palsystem.com/), coupled to an Agilent 7890 A gas chromatograph - Leco Pegasus HT time-of-flight mass spectrometer (LECO, USA) in both split (1:15 and 1:50) and splitless modes (Weckwerth et al., 2004; 101:7809–14). Chromatograms were exported from Leco ChromaTOF software (version 3.25) to R. Peak detection, retention time alignment, and library matching were obtained using the TargetSearch package from Bioconductor (Cuadros-Inostroza et al., BMC Bioinformatics. 2009;10:428). Metabolites were quantified by peak intensity of a selective mass, and metabolite intensities were normalized dividing by the sum of the total ion count followed by log2 transformation.
Ion Mode:POSITIVE
Collision Energy:70 eV
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