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MB Sample ID: SA163995
Local Sample ID: | LN1_2-EV intensity |
Subject ID: | SU001832 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | SCC-9 and LN1 |
Cell Strain Details: | Primary tumor (SCC-9) and metastatic (LN1) oral cancer cell lines |
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Combined analysis:
Analysis ID | AN002859 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Agilent DB-35MS (30m x 0,32mm x 0,25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus HT TOF |
Ion Mode | POSITIVE |
Units | intensity log2 |
MS:
MS ID: | MS002652 |
Analysis ID: | AN002859 |
Instrument Name: | Leco Pegasus HT TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | GC-TOF-MS data were obtained using a PAL-Combi XT autosampler (PAL System, Switzerland, http://www.palsystem.com/), coupled to an Agilent 7890 A gas chromatograph - Leco Pegasus HT time-of-flight mass spectrometer (LECO, USA) in both split (1:15 and 1:50) and splitless modes (Weckwerth et al., 2004; 101:7809–14). Chromatograms were exported from Leco ChromaTOF software (version 3.25) to R. Peak detection, retention time alignment, and library matching were obtained using the TargetSearch package from Bioconductor (Cuadros-Inostroza et al., BMC Bioinformatics. 2009;10:428). Metabolites were quantified by peak intensity of a selective mass, and metabolite intensities were normalized dividing by the sum of the total ion count followed by log2 transformation. |
Ion Mode: | POSITIVE |
Collision Energy: | 70 eV |