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MB Sample ID: SA167164

Local Sample ID:Celecoxib_HFD_7
Subject ID:SU001872
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL6/J
Age Or Age Range:6-7 weeks
Gender:Male
Animal Housing:temperature-controlled room under specific-pathogen free (SPF) or standard animal housing conditions with free access to food and water
Animal Feed:semi-purified normal chow diet (control fat diet (CFD), 7% w/w fat and 16% total energy from fat; AIN93G, Specialty Feeds Pty Ltd, Australia) or high fat diet (HFD, 36% w/w fat and 59% total energy from fat; SF03-002, Specialty Feeds Pty Ltd, Australia)
Animal Water:ad libitum

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Combined analysis:

Analysis ID AN002915 AN002916
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units height height

MS:

MS ID:MS002707
Analysis ID:AN002915
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Pos and neg data were acquired during the same chromatographic run using polarity switching. Full scan acquisition mode, resolution 140k, AGC target 3e6, max IT 200 ms, mass range 140-2000 m/z, number of scans 1
Ion Mode:POSITIVE
Mass Accuracy:3 ppm
Automatic Gain Control:3e6
Desolvation Gas Flow:34
Interface Voltage:3.5 kV
  
MS ID:MS002708
Analysis ID:AN002916
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Pos and neg data were acquired during the same chromatographic run using polarity switching. Full scan acquisition mode, resolution 140k, AGC target 3e6, max IT 200 ms, mass range 140-2000 m/z, number of scans 1
Ion Mode:NEGATIVE
Mass Accuracy:3 ppm
Automatic Gain Control:3e6
Desolvation Gas Flow:34
Interface Voltage:3.5 kV
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