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MB Sample ID: SA171584
| Local Sample ID: | Medici_B-1513_047_posHILIC__022 |
| Subject ID: | SU001921 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002987 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | EI |
| MS instrument type | TTOF |
| MS instrument name | ABI Sciex 6600 TripleTOF |
| Ion Mode | UNSPECIFIED |
| Units | normalized peak height |
MS:
| MS ID: | MS002777 |
| Analysis ID: | AN002987 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | TTOF |
| MS Type: | EI |
| MS Comments: | The LC/QTOFMS analyses are performed using an Agilent 1290 Infinity LC system (G4220A binary pump, G4226A autosampler, and G1316C Column Thermostat) coupled to a SCIEX Triple TOF mass spectrometer. Polar compounds are separated on an Acquity UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm maintained at 45°C at a flow-rate of 0.4 mL/min. Solvent pre-heating (Agilent G1316) was used. The mobile phases consist of: Water, 10 mM Ammonium Formate, 0.125% Formic Acid (A) and Acetonitrile: Water (95/5, v/v), 10 mM Ammonium Formate, 0.125% Formic Acid (B) The gradient is as follows: 0 min 100% (A); 0–2 min 100% (A); 2–7.7 min 30% (A); 7.7–9.5 min 60% (A); 9.5–10.3 min 70% (A); 10.3–12.8 min 0% (A); 12.8–16.8 min 0% (A. A sample volume of 1 µL for positive mode and 3 µL for negative mode is used for the injection. Sample temperature is maintained at 4°C in the autosampler. SCIEX Triple TOF 6600mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 50–1200. Number of cycles in MS1 is 1667 with cycle time of 500ms and accumulation time 475ms. Mass spectrometer parameters are as follows (positive mode) Gas Temp 300°C, gas pressures in psi units with : GS1 and GS2 50 psi, CUR: 35. ISVF is 4500V and DP and CE are 10V and 100 V. |
| Ion Mode: | UNSPECIFIED |
