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MB Sample ID: SA177880
| Local Sample ID: | MDAMB231_3_2 |
| Subject ID: | SU001998 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003119 | AN003120 | AN003121 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | Agilent 7890A | automated nanoinfusion/nanospray source | automated nanoinfusion/nanospray source |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) | None | None |
| MS Type | EI | ESI | ESI |
| MS instrument type | Triple axis detector | Ion trap | Ion trap |
| MS instrument name | Agilent 5975C | Thermo LTQ XL | Thermo LTQ XL |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | relative intensity/µg proteins | relative intensity/µg proteins | relative intensity/µg proteins |
MS:
| MS ID: | MS002900 |
| Analysis ID: | AN003119 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Triple axis detector |
| MS Type: | EI |
| MS Comments: | The initial oven temperature was set at 70 °C and then increased to 190°C (5 °C/min), 240 °C (6 °C/min), and 280 °C (5 °C/min). Data was processed using Expressionist® MSX software (version 2013.0.39, Genedata, Basel, Switzerland). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002901 |
| Analysis ID: | AN003120 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | The raw data files (*.raw) were transformed to *.mzXML using the ProteoWizard MSConvert. Data was processed using Expressionist® MSX software (version 2013.0.39, Genedata, Basel, Switzerland). |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 200 °C |
| Capillary Voltage: | 20 V |
| Gas Pressure: | 0.4 psi |
| Ion Spray Voltage: | 1.4 kV |
| MS ID: | MS002902 |
| Analysis ID: | AN003121 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | The raw data files (*.raw) were transformed to *.mzXML using the ProteoWizard MSConvert. Data was processed using Expressionist® MSX software (version 2013.0.39, Genedata, Basel, Switzerland). |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 200 °C |
| Capillary Voltage: | −2 V |
| Gas Pressure: | 0.6 psi |
| Ion Spray Voltage: | 1.7 kV |
