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MB Sample ID: SA181885
| Local Sample ID: | HFD-PB2 |
| Subject ID: | SU002013 |
| Subject Type: | Mammal |
| Subject Species: | Macaca fascicularis |
| Taxonomy ID: | 9541 |
| Age Or Age Range: | 16.3 - 18.8 yo |
| Weight Or Weight Range: | 5.73 - 10.56 kg |
| Gender: | Male |
| Animal Animal Supplier: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
| Animal Housing: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
| Animal Light Cycle: | 12-h/12-h light-dark cycle |
| Animal Feed: | normal food (19.26 % protein, 5% fat, no sugar, and no cholesterol), or high-fat and high-sugar (HFHS) food (≥ 30% sugar, ≥ 15% fat, ≥ 10.5% protein, and ≥ 0.5% cholesterol) |
| Animal Inclusion Criteria: | spontaneous diabetes mellitus and HFHS food-fed |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003145 | AN003146 | AN003147 | AN003148 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | EI | EI | EI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area | Peak area | Peak area |
MS:
| MS ID: | MS002925 |
| Analysis ID: | AN003145 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002926 |
| Analysis ID: | AN003146 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002927 |
| Analysis ID: | AN003147 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002928 |
| Analysis ID: | AN003148 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
| Ion Mode: | NEGATIVE |
