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MB Sample ID: SA185578
| Local Sample ID: | Pooled Human Plasma 7 |
| Subject ID: | SU002061 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Pooled |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002856 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters Acquity BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 |
| Ion Mode | POSITIVE |
| Units | Total Intensity Units |
MS:
| MS ID: | MS003005 |
| Analysis ID: | AN002856 |
| Instrument Name: | Agilent 6545 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Instrument settings for positive ionization mode following electrospray ionization were as follows: Sheath gas flow rate, 11 L/min; Drying gas, 8 L/min; Drying gas temperature, 320 ºC; Nebulizer, 35 psi; Capillary voltage, 3500 V; Nozzle voltage, 1000 V; Fragmentor, 175 V; Skimmer, 65 V; Octupole 1 RF Vpp, 750 V; Collision energy, 20; Iterative MS/MS mass error tolerance, ± 20 ppm; Iterative MS/MS retention time exclusion tolerance, ± 0.5 min; Spectrum data type, centroid. Data de-pendent MS/MS parameters: Mass range, 25-1200 m/z; Rate, 2 spectra/s; Max precursor ions per cycle, 3; Absolute precursor threshold, 5000 counts; Relative precursor threshold, 0.001%; Active exclusion enabled after 2 spectra and released after 0.5 min; Isolation width, narrow (~1.3 m/z). Iterative LC-MS/MS data acquired from the modified HILIC separation conditions was loaded into a pre-release version of an in-house software tool, MetIDTracker for feature detection. This software uses MSPepSearch (NIST) for database searching and score assignment. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3500 V |
| Collision Energy: | 20 |
| Collision Gas: | N2 |
| Dry Gas Flow: | 8L/min |
| Dry Gas Temp: | 320 C |
| Fragment Voltage: | 175 V |
| Fragmentation Method: | CID |
| Ionization: | ESI |
| Mass Accuracy: | <20 ppm |
