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MB Sample ID: SA196502

Local Sample ID:Fer1-2
Subject ID:SU002164
Subject Type:Cultured cells
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Cell Biosource Or Supplier:American Type Culture Collection (ATCC)
Cell Strain Details:H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.
Cell Passage Number:3-10
Cell Counts:80-90% confluency

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Combined analysis:

Analysis ID AN003394
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu GCMS-QP2010 ultra
Column Shimadzu SH-RXI (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Shimadzu QP2010 Ultra
Ion Mode POSITIVE
Units mM

MS:

MS ID:MS003161
Analysis ID:AN003394
Instrument Name:Shimadzu QP2010 Ultra
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Raw chromatography data were obtained and processed in GC-MS Labsolution Postrun Analysis software (Shimadzu Scientific Instruments, Inc., Columbia, MD) for identification of metabolites from their electron impact mass spectra by comparison to the database (NIST14/2014/EPA/NIH) [26]. Peak integration for all metabolites and multiple searches in the mass spectral library database resulted in a final database of 52 metabolic features chosen for this analysis. To assess analytical accuracy and precision, an external quality evaluation was performed using 2-fluorobiphenyl spiked into derivatization blank samples before running on the GC-MS. The quantitative analysis corresponding to the metabolite concentrations in each sample was calculated based on the internal standard in mM. A table in the format of comma-delimited (* csv) was created and uploaded to MetaboAnalyst.ca.
Ion Mode:POSITIVE
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