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MB Sample ID: SA196527
Local Sample ID: | RX_3 |
Subject ID: | SU002164 |
Subject Type: | Cultured cells |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Cell Biosource Or Supplier: | American Type Culture Collection (ATCC) |
Cell Strain Details: | H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. |
Cell Passage Number: | 3-10 |
Cell Counts: | 80-90% confluency |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003394 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS-QP2010 ultra |
Column | Shimadzu SH-RXI (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Shimadzu QP2010 Ultra |
Ion Mode | POSITIVE |
Units | mM |
MS:
MS ID: | MS003161 |
Analysis ID: | AN003394 |
Instrument Name: | Shimadzu QP2010 Ultra |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | Raw chromatography data were obtained and processed in GC-MS Labsolution Postrun Analysis software (Shimadzu Scientific Instruments, Inc., Columbia, MD) for identification of metabolites from their electron impact mass spectra by comparison to the database (NIST14/2014/EPA/NIH) [26]. Peak integration for all metabolites and multiple searches in the mass spectral library database resulted in a final database of 52 metabolic features chosen for this analysis. To assess analytical accuracy and precision, an external quality evaluation was performed using 2-fluorobiphenyl spiked into derivatization blank samples before running on the GC-MS. The quantitative analysis corresponding to the metabolite concentrations in each sample was calculated based on the internal standard in mM. A table in the format of comma-delimited (* csv) was created and uploaded to MetaboAnalyst.ca. |
Ion Mode: | POSITIVE |