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MB Sample ID: SA204267
| Local Sample ID: | WT_2 |
| Subject ID: | SU002208 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003476 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | ACQUITY UPLC |
| Column | Waters ACQUITY UPLC BEH Amide |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | AU |
MS:
| MS ID: | MS003237 |
| Analysis ID: | AN003476 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | LC-MS data was acquired on SCIEX 5500 QQQ mass spectrometer (Applied Biosystems, Foster City, CA, USA) coupled with high-performance liquid chromatography (HPLC) system ACQUITY UPLC (Waters, Milford, MA, USA). Chromatographic separation was performed on a ACQUITY UPLC BEH Amide Column (1.7 µm, 2.1 mm x 100 mm, Waters, Milford, MA, USA). The mobile phase (A) was 10 mM ammonium formate and (B) acetonitrile. The gradient program started at 85% (B) and was reduced to 70% (B) over 9 min, then reduced from 70% (B) to 30% (B) over 3 min, followed by wash with 30% (B) for another 3 min, and finally 5 min re-equilibration with 85% (B). The flow rate was 0.35 mL/min and column compartment temperature was 40ºC. The total run time was 20 min with an injection sample volume of 5 µL. |
| Ion Mode: | UNSPECIFIED |
