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MB Sample ID: SA205470
| Local Sample ID: | 1cCre |
| Subject ID: | SU002225 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN003501 | AN003502 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Ion Chromatography | None (Direct infusion) |
| Chromatography system | ThermoDionexICS3000 | TriVersa NanoMate |
| Column | ThermoDionexAS11/AG11 | TriVersa NanoMate |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 3200 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | mol% | pmol/10E6 cells |
MS:
| MS ID: | MS003261 |
| Analysis ID: | AN003501 |
| Instrument Name: | ABI Sciex 3200 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectrometric analysis was performed using the QTRAP 3200 (SCIEX) operated by Analyst 1.6.2. The electrospray ionization source parameters were −4,500 eV at 600°C, N2 gas pressures were 20 p.s.i. (curtaingas), 30 p.s.i. (gas1), and 20 p.s.i. (gas2), and collision gas was set to medium. The dwell time for ions was 75 ms, and scan time per cycle was 3.7 s.Scan ranges were from mass-to-charge ratio 87 to 606 (precursor ions) and mass-to-charge ratio 59 to 385 (product ions). Masstransitions (metabolite m/z mother ion / m/z daughter ion)recorded were as follows: UDP-glucose 565 / 323, glucose-1-phosphate 259 / 79, glucose-6-phosphate 259 / 97, 3-phosphoglycerate 185 / 97, phosphoenol pyruvate 167 / 79, citrate 191 / 87, isocitrate 191 / 111, malate 133 / 71, AMP 346/79, ADP 427/79, 2-oxoglutarate (aKG) 145 / 101, succinate 117 / 73, UDPNAG 606/385, Itaconate 129/85, Lactat 89/43, D-glucose 179/89, fumarate 115 / 71, E4P 199/97, ATP 427/79, UDP 404/79, G16BP 339/97 The contents of metabolites were calculated based on peak areas for precursor/product ion transitions relative to standards. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003262 |
| Analysis ID: | AN003502 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectrometric analysis was performed using the QTRAP 6500 (SCIEX) operated by Analyst 1.6.3. The following instrument dependent settings were used: curtain gas, 20 psi; CAD gas, medium; and interface heater temperature, 100°C. PC analysis was performed in the positive ion mode by scanning for precursors of m/z 184 at a collision energy of 35 eV. PE, PS, PG, PI, and PA measurements were performed in the positive ion mode by scanning for neutral losses of 141, 185, 189, 277, and 115 D at CE of 25 eV. The value for the declustering potential was 100 V (Özbalci et al. (2013) Methods Mol Biol 1033:3-20). Scanning was performed in a mass range of m/z 650–900 D and at a scan rate of 200 D/s. 61 MCA spectra were accumulated. Mass spectra were processed by the LipidView Software Version 1.2 (SCIEX) for identification and quantification of glycerophospholipids. Endogenous glycerophospolipids were quantified by referring their peak areas to those of the internal standards. |
| Ion Mode: | POSITIVE |
