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MB Sample ID: SA210582
| Local Sample ID: | 2 |
| Subject ID: | SU002283 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003596 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | AB SCIEX QTRAP 5500 |
| Column | Waters ACQUITY UPLC BEH C18 (75 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/mL |
MS:
| MS ID: | MS003351 |
| Analysis ID: | AN003596 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | For LC part: Mobile phase A: 1000 mL water + 2 mL formic acid (FA) Mobile phase B: 500 mL ACN + 1 mL FA Wash solvent 1: 500 mL ACN + 200 mL MeOH + 150 mL iPrOH + 150 mL water + 2.5 mL FA Wash solvent 2: water Install column and precolumn (if required). Wash the column at 95% B and condition to 100% For FIA part: Deactivate “Use flat files for scan data” in the Analyst software FIA mobile phase: FIA Mobile Phase Additive (1x glass ampule) + 290 mL MeOH Wash solvent 1: 500 mL ACN + 200 mL MeOH + 150 mL iPrOH + 150 mL water + 2.5 mL FA Wash solvent 2 (if possible): 333 mL MeOH + 333 mL iPrOH + 333 mL water For data processing: Adjust the retention times in the quantitation method and if necessary some integration parameters. Process the LC-MS data using the Quantitation Wizard. Save the results. Import the results export (TXT file) into MetIDQTM Parse the FIA-MS WIFF files directly into MetIDQTM. Evaluate results, perform statistics (e.g. using MetaboINDICATOR™, StatPack, MetaboAnalyst). |
| Ion Mode: | POSITIVE |
