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MB Sample ID: SA229508
| Local Sample ID: | KO 40 3 |
| Subject ID: | SU002414 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003796 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Dionex Ultimate 3000TM |
| Column | Millipore(TM) ZIC-pHILIC |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | intensity |
MS:
| MS ID: | MS003538 |
| Analysis ID: | AN003796 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Method duration was 30 min with polarity switching data-dependent Top 5method for both positive and negative modes. Spray voltage for both positive and negative modes was 3.5kV and the capillary temperature was set to 320 ⁰C with a sheath gas rate of 35, aux gas of 10, and max spray current of 100 μA. The full MS scan for both polarities utilized 120,000 resolution with an AGC target of 3e6 and a maximum IT of 100 ms, and the scan range was from 67-1000 m/z. Tandem MS spectra for both positive and negative modes used a resolution of 15,000, AGC target of 1 e5, maximum IT of 50 ms, isolation window of 0.4 m/z, isolation offset of 0.1 m/z, fixed first mass of 50 m/z, and 3-way multiplexed normalized collision energies (nCE) of 10, 35, 80. The minimum AGC target was 1e4 with an intensity threshold of 2e5. All data were acquired in profile mode. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | metabolomics_methods.pdf |
