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MB Sample ID: SA229524
| Local Sample ID: | A1c |
| Subject ID: | SU002415 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | N/A |
| Age Or Age Range: | 19-75 |
| Weight Or Weight Range: | N/A |
| Height Or Height Range: | N/A |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003797 | AN003798 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | area | area |
MS:
| MS ID: | MS003539 |
| Analysis ID: | AN003797 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolomics was performed by Creative Proteomics. Gingival cells enriched in immune cells were prepared by removing epithelial cells based on gradient centrifugation. HOIL samples from three control individuals were pooled and compared with three independent HIV+ individuals. Separation was performed by Ultimate 3000LC combined with Q Exactive MS (Thermo) and screened with ESI-MS (targeted MS/MS mode). The software we used for metabolites identification is Compound Discoverer 3.1 SP1. For metabolites identification, the raw data are acquired and aligned using the Compound Discover based on the m/z value and the retention time of the ion signals. CD software offers a fully integrated suite of advanced software tools for known-parent and unknown data processing and interpretation. It is used for the initial metabolites identification. We also use mzMine2 software (with parameters of m/z tolerance=0.1 m/z ) for the online database search based on the MS1 and MS2 spectra acquired. The database used is HMDB. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS_Metadata.docx |
| MS ID: | MS003540 |
| Analysis ID: | AN003798 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolomics was performed by Creative Proteomics. Gingival cells enriched in immune cells were prepared by removing epithelial cells based on gradient centrifugation. HOIL samples from three control individuals were pooled and compared with three independent HIV+ individuals. Separation was performed by Ultimate 3000LC combined with Q Exactive MS (Thermo) and screened with ESI-MS (targeted MS/MS mode). The software we used for metabolites identification is Compound Discoverer 3.1 SP1. For metabolites identification, the raw data are acquired and aligned using the Compound Discover based on the m/z value and the retention time of the ion signals. CD software offers a fully integrated suite of advanced software tools for known-parent and unknown data processing and interpretation. It is used for the initial metabolites identification. We also use mzMine2 software (with parameters of m/z tolerance=0.1 m/z ) for the online database search based on the MS1 and MS2 spectra acquired. The database used is HMDB. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS_Metadata.docx |
