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MB Sample ID: SA236622
| Local Sample ID: | TFA-T55 |
| Subject ID: | SU002443 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Age Or Age Range: | 26 weeks of age |
| Weight Or Weight Range: | 320-460 grams |
| Gender: | Male |
| Animal Animal Supplier: | RRRC (University of Missouri) |
| Animal Housing: | Center for Comparative Medicine UConn |
| Animal Feed: | Modified AIN-93G diet from Research Diets |
| Animal Water: | ab libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003842 | AN003843 | AN003844 | AN003845 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Prominence XR | Prominence XR | Prominence XR | Prominence XR |
| Column | Targa C8, 2x10 mm, 5μ | Luna C18 (150 x 2.1mm,3um) | Targa C8, 2x10 mm, 5μ | Luna C18 (150 x 2.1mm,3um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTRAP | QTRAP | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE | NEGATIVE | NEGATIVE | NEGATIVE |
| Units | ng/g tissue | mg/mL | ng/mL | mg/g tissue |
MS:
| MS ID: | MS003584 |
| Analysis ID: | AN003842 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectra for each detected lipid metabolite were recorded using the enhanced production feature to verify the identity of the detected peak. Data were collected and quantified using Analyst 1.6.2 (SCIEX) and MultiQuant (SCIEX) software, respectively. Correction for recovery efficiencies and relative quantitation of each analyte were performed using signals from each chromatogram corresponding to the spiked-in internal standards. Under standardized conditions of liquid chromatography-mass spectrometry quantitation, the detection limits for the eicosanoids are 1–2 pg on the column and the limit of quantitation is 5 pg at a signal-to-noise ratio of 3. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003585 |
| Analysis ID: | AN003843 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectra for each detected lipid metabolite were recorded using the enhanced production feature to verify the identity of the detected peak. Data were collected and quantified using Analyst 1.6.2 (SCIEX) and MultiQuant (SCIEX) software, respectively. Correction for recovery efficiencies and relative quantitation of each analyte were performed using signals from each chromatogram corresponding to the spiked-in internal standards. Under standardized conditions of liquid chromatography-mass spectrometry quantitation, the detection limits for the eicosanoids are 1–2 pg on the column and the limit of quantitation is 5 pg at a signal-to-noise ratio of 3. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003586 |
| Analysis ID: | AN003844 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectra for each detected lipid metabolite were recorded using the enhanced production feature to verify the identity of the detected peak. Data were collected and quantified using Analyst 1.6.2 (SCIEX) and MultiQuant (SCIEX) software, respectively. Correction for recovery efficiencies and relative quantitation of each analyte were performed using signals from each chromatogram corresponding to the spiked-in internal standards. Under standardized conditions of liquid chromatography-mass spectrometry quantitation, the detection limits for the eicosanoids are 1–2 pg on the column and the limit of quantitation is 5 pg at a signal-to-noise ratio of 3. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003587 |
| Analysis ID: | AN003845 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectra for each detected lipid metabolite were recorded using the enhanced production feature to verify the identity of the detected peak. Data were collected and quantified using Analyst 1.6.2 (SCIEX) and MultiQuant (SCIEX) software, respectively. Correction for recovery efficiencies and relative quantitation of each analyte were performed using signals from each chromatogram corresponding to the spiked-in internal standards. Under standardized conditions of liquid chromatography-mass spectrometry quantitation, the detection limits for the eicosanoids are 1–2 pg on the column and the limit of quantitation is 5 pg at a signal-to-noise ratio of 3. |
| Ion Mode: | NEGATIVE |
