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MB Sample ID: SA000758
| Local Sample ID: | 104013 |
| Subject ID: | SU000030 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 0.25 years |
| Gender: | male |
| Animal Animal Supplier: | University of Ottawa, Canada Mary-Ellen Harper PhD, PI; |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000027 |
| Sampleprep Summary: | - |
| Sampleprep Protocol ID: | Mitochondrial Matrix Preparation |
| Extraction Method: | Extramitochondrial Buffer Preparation: The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. Pellets were resuspended in 100 ul of buffer (140 mM KCL, 20 HEPES, 5 K2HPO4, 1 EGTA, pH 7.4 at RT) |
| Organ: | skeletal muscle |
| Organ Specification: | total |
| Cell Type: | myocyte |
| Subcellular Location: | extramitochondrial buffer |
