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MB Sample ID: SA001212
| Local Sample ID: | 3786-RV |
| Subject ID: | SU000037 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 56-78 |
| Weight Or Weight Range: | Obese or Overweight |
| Gender: | Male, Female |
| Human Race: | Caucasian, African American |
| Human Trial Type: | Intervention |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000033 |
| Sampleprep Summary: | Chenomx Internal Standard solution (70 ul) and 230 ul D20 was added to each of the 88 urine sample (400 ul), vortexed for 30s, and centrifuged at 12000 rcf for 5min. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator), 100 mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µl aliquot of the supernatant was transferred into 5mm NMR tubes (Bruker-Biospin, Germany). Phenotypic pooled urine samples were made by combining 200 µl aliquots from each of the study samples belonging to the same phenotype (Progressors-Baseline, Progressors-follow up, Non-progressors-baseline, and Non-progressors-follow up). In addition, a combined phenotypic pooled sample was also prepared by using 500 µl aliquot from each of the phenotypic pooled sample. Pooled NMR samples were prepared as described above and used as quality check (QC) samples. |
| Sampleprep Protocol ID: | RTI NMR SERUM CHENOMX method |
| Sampleprep Protocol Filename: | RTI_WOA_IDEA_Metabolomics_Procedure_Feb28_2014.docx |
| Processing Method: | Dilution using a mixture of D2O and Chenomx ISTD |
| Processing Storage Conditions: | On ice |
| Extraction Method: | None |
