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MB Sample ID: SA001407
| Local Sample ID: | D2532 |
| Subject ID: | SU000039 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Average age 68.1(9.12) |
| Weight Or Weight Range: | Average BMI of 31.4(7.01) |
| Gender: | Male, Female |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000035 |
| Sampleprep Summary: | Chenomx Internal Standard solution (70 ul) and 230 ul D20 was added to each of the 36 urine sample (400 ul), vortexed for 30s, and centrifuged at 12000 rcf for 5min. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator), 100 mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 l aliquot of the supernatant was transferred into 5mm NMR tubes (Bruker-Biospin, Germany). Phenotypic pooled urine samples were made by combining 150 l aliquots from each of the study samples belonging to the same phenotype (cases and control). In addition, a combined phenotypic pooled sample was prepared by using 1000 l aliquot from each of the phenotypic pooled sample. Pooled NMR samples were prepared as described above and used as quality check (QC) samples. |
| Sampleprep Protocol Filename: | Johnston_County_Procedure_March_4_2014.docx |
| Processing Method: | Dilution using a mixture of D2O and Chenomx ISTD |
| Processing Storage Conditions: | On ice |
| Extraction Method: | None |
