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MB Sample ID: SA003643

Local Sample ID:LI_8 52_POS_RF
Subject ID:SU000088
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:approx. 15-16 weeks
Gender:male
Animal Animal Supplier:The Jackson Laboratory
Animal Feed:high fat, low fat, or chow
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000084
Sampleprep Summary:Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol.
Sampleprep Protocol Filename:RTI__BECK-LUNG_DIET_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Lung
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