Return to study ST000093 main page
MB Sample ID: SA005261
| Local Sample ID: | Patient_149_FFA_A |
| Subject ID: | SU000112 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | postmenopausal women |
| Gender: | Female |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000108 |
| Sampleprep Summary: | Metabolons standard solvent extraction method. The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
| Sampleprep Protocol Filename: | Metabolon_Methods_CASE-03-11VW.docx |
| Sample Derivatization: | 50?L for GC/MS analysis using equal parts bistrimethyl-silyl-trifluoroacetamide and solvent mixture |
