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MB Sample ID: SA005636
| Local Sample ID: | 617 |
| Subject ID: | SU000121 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Genotype Strain: | Originating from same base population as Drosophila melanogaster genetic reference panel, selected for fast (hardy) and slow (susceptible) recovery from chill coma. |
| Age Or Age Range: | 5-7 day-old adults (mated) |
| Weight Or Weight Range: | 0.17-0.77mg |
| Height Or Height Range: | NA |
| Gender: | Female |
| Animal Housing: | 25 degC |
| Animal Light Cycle: | 12L:12D |
| Animal Feed: | Standard cornmeal molasses medium |
| Animal Water: | in diet only |
| Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000117 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | sample_prep_protocol_24Sept2014.docx sample_spiking_protocol_24Sept2014.docx |
| Sampleprep Protocol Comments: | Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-7. |
| Processing Method: | lyophilization, homogenization, solvent extraction |
| Processing Storage Conditions: | store at -20C |
| Extraction Method: | modified Bligh and Dyer protocol |
| Extract Concentration Dilution: | 2:2:1.8 (methanol:chloroform:water) |
| Extract Storage: | -20C |
| Sample Resuspension: | Samples were re-suspended in 620 ?l buffer (100 mM potassium phosphate [pH 7.3], 0.1% sodium azide, 1 mM trimethylsilylproprionate [TMSP] in D2O) |
| Sample Spiking: | 6 internal standards (proline, ?-alanine, tyrosine, histidine, phosphocholine, and 3-hydroxykynurenine) added: 6 ?L of standards were added to a concentration of 2x the Chenomx-determined concentration for each metabolite |
