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MB Sample ID: SA006224
| Local Sample ID: | 041213_SentericaCells_RidAKO_R5_0035 |
| Subject ID: | SU000138 |
| Subject Type: | Bacterial cells |
| Subject Species: | Salmonella enterica;Escherichia coli |
| Taxonomy ID: | 28901|562 |
| Genotype Strain: | DM14100|Salmonella enterica subsp. enterica serovar Typhimurium str. LT2|K 12 MG1655|K 12 MG1655 ridA3::tn10 ΔyoaB624::cat STM1549-26::kan| | |yjeF::kan |
| Species Group: | Microorganism |
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Sample Preparation:
| Sampleprep ID: | SP000135 |
| Sampleprep Summary: | - |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1.1 mL of cold, degassed extraction solvent (3:1 Methanol:Water) was added to each sample. Samples were vortexed for 20 sec then placed in sonicator for 5 min, followed by another 20 sec vortex. Samples were placed in -20C for 30 minutes prior to spinning down in centrifuge at 14 rcf for 5 min. The supernatant was transferred to 1.5 mL eppendorf tubes and evaporated in the Labconco speed vac. |
| Extract Storage: | -80C |
| Sample Resuspension: | H20 + 5mM NH4OAc + 0.2% Acetic Acid |
