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MB Sample ID: SA011846
Local Sample ID: | B_M1e |
Subject ID: | SU000278 |
Subject Type: | Bacterial cells |
Subject Species: | Sinorhizobium meliloti |
Taxonomy ID: | 382 |
Genotype Strain: | Rm2011 (SU47 str-3)|SmA818|RmP3009|RmP2917 |
Human Ethnicity: | wild type|Rm2011 lack pSymA|Rm2011 lack pSymB|Rm2011 lack pSymA and pSymB | a tRNAarg and the smb20996-engA operon from pSymB were integrated to chromosome in strains RmP3009 and RmP2917 |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP000285 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Fei,_F.,_Bowdish,_D._M._E.,_&_McCarry,_B._E._(2014)._Analytical_and_Bioanalytical_Chemistry,_406,_37233733. |
Extraction Method: | cell pellet extracted with 2:2:1 MeOH/EtOH/H2O with beadbeating |
Extract Storage: | -80ºC freezer |
Sample Resuspension: | cell extracts suspended 60%v/v acetonitrile/water |
Sample Spiking: | L-methionine-d3, L-tryptophan-d5 as standards for recovery determination ; L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine as internal standards for peak area normalization |
Cell Type: | Gram negative bacterium |