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MB Sample ID: SA012002
| Local Sample ID: | P044 |
| Subject ID: | SU000281 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000289 |
| Sampleprep Summary: | Cold Methanol/H2O/Chloroform Extraction |
| Sampleprep Protocol Comments: | Extraction protocol: Step 1: Weight each intact frozen tissue and add 0.25 ml methanol and 0.053 ml deionized water for each sample, after homogenizing in ice bath using the Tissue Tearor (Model 985-370, BioSpec Products, Inc.), then following by vortexing the mixture and then adding 0.125 ml chloroform, vortexing again. Step 2: 0.125 ml chloroform and 0.125 ml deionized water were added to the sample and then vortexed again, following by transferring different layers into glass vials separately with syringes after the mixture being left on ice for 15 min and centrifuged at 6,000 rpm for 16 min at 4 °C. Finally, the MeOH/H2O layer solvents (hydrophilic metabolites) were removed by employing the lyophilizer. And the extracts were stored at ?80 °C freezer before performing NMR measurements. |
