Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA012513

Local Sample ID:	155
Subject ID:	SU000304
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	18-88
Gender:	Femal(116), Male(118)
Human Smoking Status:	67 nonsmokers and 167 smokers
Human Alcohol Drug Use:	14 no alcohol and 220 alcohol
Species Group:	Human

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000312
Sampleprep Summary:	Frozen samples were first thawed at room temperature (25 °C) and 50 uL of each serum sample was placed in a 2 Scientific). The initial step for extraction was performed vortexed centrifuged at 20 800g for 10 min, and the supernatant was Eppendorf vial. To the first vial containing methanol was added, and the metabolite the supernatant was collected in the same vial that supernatant. The resulting supernatants dried using a Vacufuge dried mM ammonium acetate in 40% water/60% acetonitrile 5.13 ?M L-tyrosine-13C2 and 22.5 Laboratory). each through 0.45 ?m PVDF filters (Phenomenex, Torrance, analysis. A pooled sample, which was a polyp patients, and as sample and was analyzed once every 10 patient

Sampleprep ID:

SP000312

Sampleprep Summary:

Frozen samples were first thawed at room temperature (25 °C) and 50 uL of each serum sample was placed in a 2 Scientific). The initial step for extraction was performed vortexed centrifuged at 20 800g for 10 min, and the supernatant was Eppendorf vial. To the first vial containing methanol was added, and the metabolite the supernatant was collected in the same vial that supernatant. The resulting supernatants dried using a Vacufuge dried mM ammonium acetate in 40% water/60% acetonitrile 5.13 ?M L-tyrosine-13C2 and 22.5 Laboratory). each through 0.45 ?m PVDF filters (Phenomenex, Torrance, analysis. A pooled sample, which was a polyp patients, and as sample and was analyzed once every 10 patient